Bovine Serum Albumin (IgG-Free, Protease-Free) from Jackson ImmunoResearch Laboratories, Inc.

Product Specs
ItemBovine Serum Albumin (IgG-Free, Protease-Free)
CompanyJackson ImmunoResearch Laboratories, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Number001-000-162
Quantity50 g
SpeciesBovine
Conjugate/Tag/LabelUnconjugated
Molecule NameSerum Albumin (IgG-Free, Protease-Free)
FormatBSA
PurityTested negative for bovine IgG and for protease

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Jackson ImmunoResearch Laboratories, Inc.

872 West Baltimore Pike

West Grove, Pennsylvania 19390

United States

Phone: 1-800-FOR-JAXN (367-5296)
Phone: 610-869-4024
Fax: 610-869-0171

Email: cuserv@jacksonimmuno.com

Company Profile

Website: www.jacksonimmuno.com

(1) Limitations of PLX3397 as a microglial investigational tool: peripheral and off-target effects dictate the response to inflammationFrontiers in ImmunologyNovember 22, 2023Wouter Claeys, Daan Verhaege, Griet Van Imschoot, Elien Van Wonterghem, Lore Van Acker, Laura Amelinck, Federico F. De Ponti, Charlotte Scott, Anja Geerts, Christophe Van Steenkiste, et al.supernatans were aspirated and the pellet was resuspended in 50 µl of 0.5% BSA (Jackson ImmunoResearch, 001‐000‐162) in D‐PBS (Gibco 14190‐094). This process was repeated twice. Single cell suspensions were kept on ice

(2) Oxytocinergic projection from the hypothalamus to supramammillary nucleus drives recognition memory in micePLOS ONENovember 16, 2023Junpei Takahashi, Daisuke Yamada, Wakana Nagano, Yoshitake Sano, Teiichi Furuichi, Akiyoshi SaitohSubsequently, sections were washed using PBST three times and blocked with blocking solution [3% BSA (catalog #001-000-162, Lot; 90246 Jackson ImmunoResearch, Pennsylvania, USA) in PBST] for 1 h. Then, the sections were incubated

(3) Deep learning-driven adaptive optics for single-molecule localization microscopyNature MethodsNovember 1, 2023Peiyi Zhang, Donghan Ma, Xi Cheng, Andy P. Tsai, Yu Tang, Hao-Cheng Gao, Li Fang, Cheng Bi, Gary E. Landreth, Alexander A. Chubykin, et al.(452882, Sigma-Aldrich) in 1× PBS. The cells were rinsed three times with 1× PBS and blocked with 3% BSA (001-000-162, Jackson ImmunoResearch) and 0.2% Triton X-100 in 1× PBS, with gently rocking at RT for 1 h. After blocking,

(4) Liquid biopsy epigenomic profiling for cancer subtypingNature MedicineNovember 1, 2023Sylvan C. Baca, Ji-Heui Seo, Matthew P. Davidsohn, Brad Fortunato, Karl Semaan, Shahabbedin Sotudian, Gitanjali Lakshminarayanan, Miklos Diossy, Xintao Qiu, Talal El Zarif, et al.protein G (Invitrogen, 10004D) for at least 6 h at 4 °C with rotation in 0.5% BSA (Jackson Immunology, 001-000-161) in PBS (Gibco, 14190250), followed by blocking with 1% BSA in PBS for 1 h at 4 °C with rotation. The

(5) Pax7 haploinsufficiency impairs muscle stem cell function in Cre-recombinase mice and underscores the importance of appropriate controlsStem Cell Research & TherapyOctober 13, 2023Despoina Mademtzoglou, Perla Geara, Philippos Mourikis, Frederic Relaixwith 0.5% Triton-X (Sigma) for 8 min, washed with PBS, then blocked with 5% BSA (Jackson Laboratories 001–000-162) for 1 h at room temperature. Sections were then incubated with the primary antibodies overnight at

(6) The nanoscale organization of reticulon 4 shapes local endoplasmic reticulum structure in situJournal of Cell BiologyOctober 2, 2023Lukas A. Fuentes, Zach Marin, Jonathan Tyson, David Baddeley, Joerg Bewersdorfbuffer (0.3% IGEPAL CA-630 [I8896; Sigma-Aldrich], 0.05% Triton X-100 [T8787; Sigma-Aldrich], 0.1% BSA [001-000-162; Jackson ImmunoResearch] in 1× PBS) for 3 min followed by another three washes with 1× PBS. Samples

(7) Heterogeneous receptor expression underlies non-uniform peptidergic modulation of olfaction in DrosophilaNature communicationsAugust 30, 2023Sizemore TR, Jonaitis J, Dacks AM.ascending-descending ethanol wash series, then blocking in 4% IgG-free BSA (Jackson Immunoresearch; Cat#001-000-162). Samples were then incubated in primary antibody (Table 1) diluted in blocking solution and 5 mM sodium

(8) Helicase-like transcription factor (Hltf)-deletion activates Hmgb1-Rage axis and granzyme A-mediated killing of pancreatic β cells resulting in neonatal lethalityPLoS OneAugust 25, 2023Gurvinder Kaur, Rebecca A. Helmer, Dalia Martinez-Marin, Souad R. Sennoune, Rachel L. Washburn, Raul Martinez-Zaguilan, Jannette M. Dufour, Beverly S. ChiltonBeckman Coulter Life Sciences (Indianapolis, IN). IgG-free, protease-free bovine serum albumin (BSA, 001-000-162) was purchased from Jackson ImmunoResearch (West Grove, PA). Primary and secondary antibodies used with

(9) Spatiotemporally organized immunomodulatory response to SARS-CoV-2 virus in primary human broncho-alveolar epitheliaiScienceAugust 18, 2023Diana Cadena Castaneda, Sonia Jangra, Marina Yurieva, Jan Martinek, Megan Callender, Matthew Coxe, Angela Choi, Juan García-Bernalt Diego, Jianan Lin, Te-Chia Wu, et al.serum,Jackson ImmunoResearch,Cat#015-000-120 "BSA (IgG-Free, Protease-Free)",Jackson ImmunoResearch,Cat#001-000-161 Dimethyl Sulfoxide (DMSO),Sigma,Cat#67-68-5 Trypsin-EDTA (0.05%) phenol red,Thermo Fisher,Cat#25-300-062 Zombie

(10) ATF3 induction prevents precocious activation of skeletal muscle stem cell by regulating H2B expressionNat CommunAugust 17, 2023Suyang Zhang, Feng Yang, Yile Huang, Liangqiang He, Yuying Li, Yi Ching Esther Wan, Yingzhe Ding, Kui Ming Chan, Ting Xie, Hao Sun, et al.acid (pH 6.0) was performed for 5 min in a microwave. After 4% BBBSA (4% IgG-free BSA in PBS; Jackson, 001-000-162) blocking, the sections were further blocked with unconjugated AffiniPure Fab Fragment (1:100 in PBS;

(11) Mitochondria dysregulation contributes to secondary neurodegeneration progression post-contusion injury in human 3D in vitro triculture brain tissue modelCell death & diseaseAugust 3, 2023Liaudanskaya V, Fiore NJ, Zhang Y, Milton Y, Kelly MF, Coe M, Barreiro A, Rose VK, Shapiro MR, Mullis AS, et al.minutes at room temperature in 5% BSA or 5% non-fat dry milk (NFDM) (Jackson Immuno Research Labs, 001-000-161) in TBST (Boston BioProducts, IBB-181) and then incubated with primary antibodies diluted in 5% BSA

(12) Different activity patterns control various stages of Reelin synthesis in the developing neocortexCerebral CortexJuly 24, 2023Kira Engeroff, Davide Warm, Stefan Bittner, Oriane Blanquiea goat Reelin antibody (AF3820, R&D Systems, 1:500) diluted in PBS 0.01 M/2% bovine serum albumin (001-000-161, Jackson Immunoresearch)/0.05% sodium azide (S002, Sigma-Aldrich)/0.1% Triton. The next day, slices

(13) RAD21 is the core subunit of the cohesin complex involved in directing genome organizationGenome BiologyJune 28, 2023Yuao Sun, Xin Xu, Wenxue Zhao, Yu Zhang, Keyang Chen, Yongzheng Li, Xiaotian Wang, Mengling Zhang, Boxin Xue, Wanting Yu, et al.T8787-50 ML) in PBS for 10 min, followed by blocking with 5% IgG-free bovine serum albumin (BSA, Jackson, 001-000-162) for 30 min. The indicated primary antibody was added to 5% BSA in PBS at an appropriate dilution and

(14) Chronic social defeat alters brain vascular-associated cell gene expression patterns leading to vascular dysfunction and immune system activationJournal of NeuroinflammationJune 28, 2023Joshua D. Samuels, Madison L. Lotstein, Michael L. Lehmann, Abdel G. Elkahloun, Subhadra Banerjee, Miles Herkenhamml of 1X phosphate buffered saline (PBS) with 1% bovine serum albumin (BSA; Jackson ImmunoResearch, 001-000-162) and 2 mM etheylenediaminetetraacetic acid (EDTA; 0.5 M pH 8.0, Invitrogen AM9260G; full solution referred

(15) Activity-dependent regulation of the BAX/BCL-2 pathway protects cortical neurons from apoptotic death during early developmentCellular and molecular life sciences : CMLSJune 3, 2023Schroer J, Warm D, De Rosa F, Luhmann HJ, Sinning A.2 h at RT. Afterward, cells were incubated overnight at 4 °C in PBS 0.01 M/2% bovine serum albumin (001-000-161, Jackson ImmunoResearch)/0.05% sodium azide (S002, Sigma-Aldrich)/0.1% triton with the following primary

(16) Experimental hepatic encephalopathy causes early but sustained glial transcriptional changesJournal of NeuroinflammationMay 29, 2023Wouter Claeys, Lien Van Hoecke, Hannah Lernout, Clint De Nolf, Griet Van Imschoot, Elien Van Wonterghem, Daan Verhaege, Jonas Castelein, Anja Geerts, Christophe Van Steenkiste, et al.and the pellet was resuspended in 50 µl of 0.5% bovine serum albumin (BSA) (Jackson ImmunoResearch, 001‐000‐162) in D‐PBS (Gibco 14190‐094). This process was repeated twice. Single cell suspensions were pre-incubated

(17) Phenotypic diversity of T cells in human primary and metastatic brain tumors revealed by multiomic interrogationNature CancerMay 22, 2023Vladimir Wischnewski, Roeltje R. Maas, Paola Guerrero Aruffo, Klara Soukup, Giovanni Galletti, Mara Kornete, Sabine Galland, Nadine Fournier, Johanna Lilja, Pratyaksha Wirapati, et al.Tween, 10% donkey serum (Sigma-Aldrich, S30-M) and 2% bovine serum albumin (Jackson ImmunoResearch, 001-000-162) filtered through a 0.22-µm filter. At the end of this incubation period, the blocking buffer was replaced

(18) The nanoscale organization of reticulon 4 shapes local endoplasmic reticulum structure in situbioRxivMay 20, 2023Lukas A. Fuentes, Zach Marin, Jonathan Tyson, David Baddeley, Joerg Bewersdorf[Sigma-Aldrich; I8896], 0.05% Triton X-100 [Sigma-Aldrich; T8787], 0.1% BSA [Jackson ImmunoResearch; 001–000-162] in 1xPBS) for 3 minutes followed by another three washes with 1xPBS. Samples were blocked for 1 hour

(19) Differential Functional Contribution of BK Channel Subunits to Aldosterone-Induced Channel Activation in Vascular Smooth Muscle and Eventual Cerebral Artery DilationInternational Journal of Molecular SciencesMay 12, 2023Steven C. Mysiewicz, Sydney M. Hawks, Anna N. Bukiya, Alex M. Dopico(T8961; SLBP8056V; St. Louis, MO, USA) were obtained from Sigma-Aldrich. BSA (Jackson ImmunoResearch; 001-000-162; 153078; West Grove, PA, USA), papain (MP Biomedicals; 100921; S6808; Solon, OH, USA), and soybean trypsin

(20) Voltage sensor current, SR Ca2+ release, and Ca2+ channel current during trains of action potential‐like depolarizations of skeletal muscle fibersPhysiological ReportsMay 5, 2023Hugo Bibollet, Elton L. Nguyen, Daniel R. Miranda, Christopher W. Ward, Andrew A. Voss, Martin F. Schneider, Erick O. Hernández‐OchoaNo. 21083027) supplemented with 0.25% w/v bovine serum albumin (BSA; Jackson ImmunoResearch, Cat. No. 001–000‐161). Then, the fibers were washed thoroughly with plain L‐15 media to remove residual fluorescent dye.

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Bovine Serum Albumin (IgG-Free, Protease-Free) from Jackson ImmunoResearch Laboratories, Inc.

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Jackson ImmunoResearch Laboratories, Inc.

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Bovine Serum Albumin (IgG-Free, Protease-Free) from Jackson ImmunoResearch Laboratories, Inc.

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